Simultaneous Quantification of 17 Cannabinoids in Cannabis Inflorescence by Liquid Chromatography-Mass Spectrometry

With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand accurate and reliable quantification a growing number them. Although recent methods are based on highly selective chromatography-mass spectrometry technology, most limited to few cannabinoids, while relying unnecessarily sophisticated expensive ultra-high performance liquid chromatography tandem mass spectrometry. Here we report optimised, simple extraction method followed by high separation. The detection performed using time-of-flight spectrometer that available in natural products research laboratories. Due simplicity instrumentation, robustness resulting from resolution isobaric well suited routine phytocannabinoid analysis range applications. was validated terms limits, repeatability, recoveries total 17 cannabinoids: limits were 11–520 pg when 1 µL sample injection volume, recovery percentages ranged 85% 108%. subsequently applied determine cannabinoid composition inflorescences several medicinal sativa varieties.